NAD-linked Isocitrate Dehydrogenase

Reactants and Reaction

NAD-linked isocitrate dehydrogenase catalyzes the fourth step in the oxidation of acetyl-CoA in the citric acid cycle. The reference chemical reaction is

$$\text{NAD}^{+} + \text{ICIT}^{3-} + \text{H}_{2}\text{O}\rightleftharpoons\text{AKG}^{2-} + \text{NADH}^{0} + \text{CO}^{2-}_{3} + 2\text{H}^{+}$$

where the abbreviations for the biochemical species are listed below. The biochemical reaction, involving biochemical reactants that are sums of species, is

$$\text{NAD} + \text{ICIT} \rightleftharpoons\text{AKG} + \text{NADH} + \text{CO}_{2}\text{tot}$$

Chen X. Qi F., and Beard D.A. Detailed kinetics and regulation of mammalian NAD-linked isocitrate dehydrogenase. (manuscript in review), 2008.