Citrate Synthase

Reactants and Reaction


Citrate synthase is the first step in the oxidation of acetyl-CoA in the citric acid cycle. The reference chemical reaction, is


$$\text{OAA}^{2-} + \text{ACCOA}^0 + \text{H}_2\text{O} \rightleftharpoons \text{COAS}^- + \text{CIT}^{3-} + 2\text{H}^+$$


where the abbreviations for the biochemical species are listed below. The biochemical reaction, involving biochemical reactants that are sums of species is


$$ \text{OAA} + \text{ACCOA} \rightleftharpoons \text{COASH} + \text{CIT}$$


Beard, DA, KC Vinnakota, and F Wu. Detailed enzyme kinetics of biochemical species: study of citrate synthase. PLoS One 3(3):e1825, 2008.